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Image Search Results
Journal: bioRxiv
Article Title: Evolutionary engineering a larger porin using a loop-to-hairpin mechanism
doi: 10.1101/2023.06.14.544993
Figure Lengend Snippet: (A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Article Snippet: The following antibodies were diluted as per manufacturer recommendation with TBST with 1% gelatin: THE
Techniques: Western Blot, Membrane, Staining, Recombinant, Positive Control
Journal: bioRxiv
Article Title: Evolutionary engineering a larger porin using a loop-to-hairpin mechanism
doi: 10.1101/2023.06.14.544993
Figure Lengend Snippet: (A) Single channel conductance of PorB 18 (blue) compared to PorB WT (green). (B) Box and whisker plot comparing the conductance of PorB WT (green) and PorB 18 (blue). ****: p-value <= 10 -4 (C) Graph shows comparison of experimental single-channel conductance of PorB WT with His-Tag (green) and without His-tag (light green), PorB 18 (blue), theoretical shingle-channel conductance of AlphaFold 18-stranded model (magenta) and 16-stranded model (cyan) of PorB 18 . (D) Channel conductance at 100 mV showing channel closing for individual subunits of PorB WT (green) and PorB 18 (blue). Shortened loop constructs collapse
Article Snippet: The following antibodies were diluted as per manufacturer recommendation with TBST with 1% gelatin: THE
Techniques: Whisker Assay, Comparison, Construct